Equipment, Instrumentation, and Facilities

CIT occupies over 4000 square feet of office space, laboratory facilities, and a production floor.

Laboratory

  • Four 20-liter Chemap bioreactors. These bioreactor are fully automated and equipped with gas mixing station, and are capable of measurement and control of all parameters such as pH, dissolved oxygen, foam control, temperature, RPM, and substrate.
  • Hewlett-Packard Gas Chromatography Model 6890 plus with flame ionization detector and capillary column.
  • Zeiss Axioskop research microscope, equipped for phase contrast and fluorescent microscopy.
  • Three large Kuhner shakers Model ISF-1-W capable of controlling the temperature from 0-80°C. Platforms are available for flasks of all sizes.
  • Beckman spectrophotometer Model DU 520 capable of enzymes kinetic studies.
  • Beckman Centrifuge Model Allegra 6.
  • Vacuum distillation setup from 500 ml to 5000 ml
  • Buchi Rotavaper model R-124.
  • Fractional distillation up to 22 liter.
  • Baker 6 ft Biological Hood.
  • A 6 ft chemical Hood
  • Large Amsco Autoclave
  • -80°C Freezer with liquid CO2 Back-up

Production Facility

All fermenters are Chemap Brand (made in Switzerland). They were commissioned for six months by Anheuser-Busch and have been in warehouses for years. We have refurbished them from ground-up. A new modern controller with touch screen and remote computer (Eurotherm Controls) has been installed. The following is the list of the fermenters and supporting equipment:

  • Two 300 L fermenters, 7.5 hp motor, 3 Rushton Impellers, H:D Ratio 3:1, Fully automatic, with various addition lines, pH, DO2, massflowmeters, etc.
  • One 750 L fermenter, 10 hp motor, 3 Rushton Impellers, H:D Ratio 3:1, Fully automatic, with various addition lines, pH, DO2, massflowmeters, etc.
  • Two 3000 L fermenters, 30 hp motor, 4 Rushton Impellers, H:D Ratio 3:1, Fully automatic, with various addition lines, dual pH, Dual DO2, two massflowmeters, etc.
  • One 5,500 L fermenter
  • Two 10 L addition tanks, sterilizable in place, with Vibro Mixer
  • Two 30 L addition tanks, sterilizable in place, with Vibro Mixer
  • Two 250 L addition tanks, sterilizable in place, with temperature control, with Vibro Mixer
  • Four 100 L addition tanks, sterilizable in place, with Vibro Mixer
  • One 3000 L harvest tank with temperature control, lightnin agitator; also used for steam distillation

Product Recovery

  • LUWA - Wipe Film Evaporator, 1.4 square, with external condenser. Equipped with heat exchanger for preheating the product with steam and hot oil system (550°F).
  • Pfaudler - Wipe Film Evaporator, 1.2 square, internal condenser
  • 12.5 L glass still with Heli-Pak packing
  • 72 L glass still with 3 feet column, Heli-Pak packing
  • Cherry-Burrel, 250 gallon jacked tank, 316 SS, National Board Certified
  • Centrifuge-Westfalia SC-35-06-177
  • Westernstate basket centrifuge, 316 SS, 30" x 18"
  • Two FSI bag filters, 304 SS, Model BFNP12-2-2
  • Ultrafilteration unit with six Amersham model UFP500E85 UF cartridges
  • Two glass receivers, 300 mm x 1000 mm
  • Micromtion models T025T621SQBUEZZZX and CMF100M339QBUEZZZ
  • Mobile refrigeration unit 8 x 45 ft

Infrastructure

  • Boiler: Gas fired, 60 hp, 150 psi
  • Compressors: Two compressors, 25 and 20 hp with matching dryers, 150 psi, computer controlled
  • Hot oil system - 34 kW
  • Process chiller with glycol, 30,000 BTU, 15°F
  • Electricity: 400 A, 3 phase 460
  • Natural Gas: 54 inch pressure
  • Water: CANDO
  • Sewer: CANDO